Structural studies of viruses by Raman spectroscopy. Novel vibrational assignments for proteins from Raman spectra of viruses

摘要: Raman spectroscopy and ultraviolet resonance are versatile methods for probing details of protein structure dynamics in complex biological assemblies, including viruses. The information contained the spectrum a virus is ordinarily interpreted on basis an understanding that has been developed from detailed spectroscopic investigations simpler better characterized molecular structures, such as small globular proteins, peptides related model compounds their isotopic derivatives. systems approach serves generally foundation reliable band assignments key to successful application supramolecular assemblies. However, converse also possible. assembly may serve ‘model compound’ its signature provide novel spectra–structure correlations applicable isolated subunits, proteins or molecules. Thus, particle can yield new insights into vibrational assignments. Here, number have emerged studies filamentous viruses identified. bands question not identified previously demonstrated originate either vibrations main-chain (CαH marker, 1340–1350 cm-1) aromatic amino acid side chains (phenylalanine 827 cm-1; tyrosine singlet, 850–855 tryptophan 1560 cm-1). present results, which considered light existing between data spectra structure, suggest much remains be learned about structural significance bands. © 1998 John Wiley & Sons, Ltd.