Effects of chelating agent and environmental stresses on microbial biofilms: relevance to clinical microbiology

摘要: Aims:  To determine the effect of pH, temperature, desiccation, ethylenediaminetetraacetic acid (EDTA) and desferrioxamine B (DFO) on Panton-Valentine leukocidin-positive community acquired methicillin-susceptible Staphylococcus aureus (PVL +ve CA-MSSA) biofilm formation. Methods Results:  Biofilms from PVL CA-MSSA (clinical isolate) were subjected to EDTA DFO. more resistant pH heat than their planktonic equivalents. Desiccation studies demonstrated that biofilms refractory treatment cells. Significant inhibition formation was observed in presence 1 mmol l−1 EDTA. Low concentrations (2·5 μmol l−1) DFO enhanced growth biofilms. At higher (1 mmol l−1), did inhibit but not as much A combination inhibited at lower either alone. Conclusions:  This study demonstrates are environmental stress can effectively by a mixture DFO. Significance Impact Study:  The using chelating agents has been previously reported provides practical approach achieve disruption these potentially important formed an emerging pathogen.