Magnetic microspheres encoded with photoluminescent quantum dots for multiplexed detection

摘要: A combination of layer-by-layer (LBL) self-assembly and magnetic separation has been used to encode microspheres with multiple colours photoluminescent semiconductor quantum dots (QDs). Magnetic increased the speed LBL process led high yields encoded microspheres. This is important because it allows full potential QDs be harnessed for optically The assembly was investigated using fluorescence microscopy, transmission electron microscopy (TEM) microelectrophoresis. same repeated on planar surfaces atomic force (AFM) UV/vis spectroscopy. Three sets different spectral codes were combined form a suspension array that screen three types explosive in sample. Results analyzed microscope decoded detected fluorescent reporter molecules bound their surface. results allowed individual explosives mixtures multiplexed immunoassays.