Glutathione-Mediated Regulation of ATP Sulfurylase Activity, SO42- Uptake, and Oxidative Stress Response in Intact Canola Roots

摘要: The dual role of glutathione as a transducer S status (A.G. Lappartient and B. Touraine [1996] Plant Physiol 111: 147–157) an antioxidant was examined by comparing the effects deprivation, feeding, H2O2 (oxidative stress) on SO42- uptake ATP sulfurylase activity in roots intact canola (Brassica napus L.). increased rate severely decreased exposed to 10 mM H2O2, whereas both S-starved plants. In split-root experiments, oxidative stress response induced remote from exposure, revealed changes reduced (GSH) level GSH/oxidized (GSSG) ratio, but there only small decrease no effect activity. Feeding plants with GSH GSH, did not affect GSH/GSSG were inhibited. responses H2O2-scavenging enzymes ascorbate peroxidase reductase starvation, treatment, treatment glutathione-mediated demand regulatory process. We conclude that regulation is related rather than distinct response.