Karyotypic evolution and phylogenetic relationships in the order Chiroptera as revealed by G-banding comparison and chromosome painting

摘要: Bats are a unique but enigmatic group of mammals and have world-wide distribution. The phylogenetic relationships extant bats far from being resolved. Here, we investigated the karyotypic representative species four families order Chiroptera by comparative chromosome painting banding. A complete set probes derived flow-sorted chromosomes Myotis myotis (family Vespertilionidae) were hybridized onto metaphases Cynopterus sphinx (2n = 34, family Pteropodidae), Rhinolophus sinicus 36, Rhinolophidae) Aselliscus stoliczkanus 30, Hipposideridae) delimited 27, 30 25 conserved chromosomal segments in three genomes, respectively. results substantiate that Robertsonian translocation is main mode evolution Chiroptera, with extensive conservation whole arms. use M. 44) has enabled integration C. sphinx, R. A. into previously established maps between human Eonycteris spelaea 36), mehelyi 58), Hipposideros larvatus 32), myotis. Our provide first cytogenetic signature rearrangement supports grouping Pteropodidae Rhinolophoidea common clade (i.e. Pteropodiformes or Yinpterochiroptera) thus improve our understanding on genome phylogeny these bat species.