Characterization of digestive α-amylase in the midgut of willow leaf beetle Plagiodera versicolora (Coleoptera: Chrysomelidae)

摘要: Willow leaf beetle, Plagiodera versicolora is an important pest of willow trees that feeds on the leaves both as larvae and adults. -Amylases (EC 3.2.1.1) are major insect digestive enzymes catalyze endohydrolysis long - 1,4-glucan chains such starch glycogen. In current study, -amylase activity was studied in midgut adults P. versicolora. Amylase 0.6807 0.1162 µmol/min/mg protein, respectively. The optimal pH for 4 8, respectively temperature 35 °C. enzyme inhibited by addition Na 2+ , K + Zn . (at 5 mmol) had most positive effect α-amylase EGTA significant influence decreasing larvae. EDTA increasing Plant amylase inhibitors play role against pests. Therefore, characterization examination could be useful tackling