Detecting Cacopsylla pyricola (Hemiptera: Psyllidae) in predator guts using COI mitochondrial markers

摘要: Cacopsylla pyricola (Forster) is one of the most important pests pear in North America, where several native predators have been considered for integrated pest management (IPM) programmes. Two molecular markers 271 and 188 bp were developed from C. cytochrome oxidase I (COI) fragments, order to study detection this species gut arthropod predators. Primer sensitivity period psylla remains guts Anthocoris tomentosus Pericart determined. The threshold was defined at 10-5 dilution a fifth-instar nymph all samples. Predator adults evaluated immediately after ingestion five nymphs (t = 0) 2, 4, 6, 8, 16, 24 32 h. Detection presence DNA always lasted longer using shorter fragment observed h digestion both markers. primers amplifying amplified four psyllid tested, whereas designed amplify did so exclusively its close relative, pyri (Linnaeus). Both failed representative Coccinellidae, Chrysopidae, Hemerobiidae, Anthocoridae, Miridae, Salticidae, Aphididae, Tetranychidae Tortricidae, suggesting their suitability general trophic studies.