Raman molecular imaging of brain frozen tissue sections.

摘要: Raman spectroscopy provides a molecular signature of the region being studied. It is ideal for neurosurgical applications because it non-destructive, label-free, not impacted by water concentration, and can map an entire tissue. The objective this paper to demonstrate meaningful spatial information provided identification regions normal brain, necrosis, diffusely infiltrating glioma solid glioblastoma (GBM). Five frozen section tissues (1 normal, 1 necrotic, GBM, 2 glioma) were mapped in their entirety using 300-µm-square step size. Smaller interest also 25-µm relative concentrations relevant biomolecules across all compared with adjacent hematoxylin eosin-stained sections, allowing necrotic regions. peaks peak ratios included 1003, 1313, 1431, 1585, 1659 cm−1. Tissue maps identified boundaries grey white matter, tumor. Complementary information, including concentration lipids, protein, nucleic acid, hemoglobin, was presented manner which be easily adapted vivo tissue mapping. successfully provide label-free imaging characteristics high accuracy. translated surgical or laboratory tool rapid, non-destructive tumor margins.