Antibiotic resistance of aquatic bacteria and it's implications for limnological research

摘要: The antibiotic resistance of bacteria from the water column various rivers and billabongs in north-eastern Victoria was studied during 1988-91. Two indices were used as measures resistance: conventional disc-sensitivity tests with culturable bacteria, effect antibiotics on uptake 14C-glucose 14C-amino acids by native bacterial assemblages. Culturable resistant to ampicillin, methicillin penicillin but generally sensitive nalidixic acid, neomycin, streptomycin, sulfafurazole tetracycline. Responses chloramphenicol, erythromycin, nitrofurantoin sulfamethizole variable. Bacteria isolated more than those billabongs. Methicillin, even at 200 mg L-1, had little 14C-labelled compounds. Nalidic acid (20 L-1) also ineffective unless lengthy pre-incubations (24 h) used. Chloramphenicol tetracycline 20 L-1 markedly reduced amino not glucose uptake. widespread aquatic casts doubt suitability these compounds selectively inhibit prokaryotic component ecological studies may be cause for concern regarding spread through natural communities.