The use of 5'-phospho-2-deoxyribocytidylylriboadenosine as a facile route to chemical aminoacylation of tRNA

摘要: Abstract Methodology is described for the synthesis and chemical aminoacylation of hybrid dinucleotide 5'-phospho-2'-deoxyribocytidylylriboadenosine (pdCpA). Ligation aminoacylated pdCpA to a truncated amber suppressor tRNACUA (-CA) using T4 RNA ligase generates an tRNA which can be used site-specific incorporation unnatural amino acids into proteins. Both ligation in vitro suppression efficiencies are same when either pCpA or used. The use deoxycytidine simplifies chemistry involved pCpA. In addition, these results demonstrate that ribocytidine not required recognition during protein synthesis.