MicroRNA-143 targets ERK5 in granulopoiesis and predicts outcome of patients with acute myeloid leukemia.

摘要: Hematopoiesis, the formation of blood cells from hematopoietic stem (HSC), is a highly regulated process. Since discovery microRNAs (miRNAs), several studies have shown their significant role in regulation system. Impaired expression miRNAs leads to disrupted cellular pathways and particular causes loss ability. Here, we report previously unrecognized function miR-143 granulopoiesis. Hematopoietic undergoing granulocytic differentiation exhibited increased expression. Overexpression or ablation resulted accelerated block differentiation, respectively. The absence mice reduced number mature granulocytes bone marrow. Additionally, observed an association high levels with higher probability survival two different cohorts patients acute myeloid leukemia (AML). AML impaired cell growth, partially induced caused apoptosis. Argonaute2-RNA-Immunoprecipitation assay revealed ERK5, member MAPK-family, as target cells. Further, inverse correlation ERK5 primary patient samples, CD34+ HSPCs confirmed functional relevance In conclusion, our data describe relevant factor granulocyte whose may be useful prognostic therapeutic therapy.