摘要: Publisher Summary This chapter presents a procedure for the preparation of catechol O-methyltransferase that catalyzes transfer methyl group from S-adenosylmethionine (AdoMet) to substrate, resulting in formation meta- and para-O-methylated products. The convenient assay activity is radiochemical technique using either [14CH3]AdoMet or [C3H31AdoMet. 14CH3- C3H3-labeled O-methylated products are isolated by simple solvent extraction. based on use [14C]AdoMet 3,4–dihydroxybenzoic acid as substrates, followed extraction 14CHh-labeled (3-hydroxy-4-methoxybenzoic 4-hydroxy-3-methoxybenzoic acid). Catechol purified rat liver, involving subcellular fractionation, precipitation; ammonium sulfate washing sephadex G-25, an affinity chromatography system consist 3,4–dimethoxy-5-hydroxyphenylethylamine-Sepharose 4B conjugate, G-100 column.
sci-hub.se 参考文章(8)
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
F.A. Raymond, R.M. Weinshilboum, Microassay of human erythrocyte catechol-O-methyltransferase: Removal of inhibitory calcium ion with chelating resin Clinica Chimica Acta. ,vol. 58, pp. 185- 194 ,(1975) , 10.1016/S0009-8981(75)80012-X
F. Mauthner, Die Synthese der Iridinsäure Justus Liebig's Annalen der Chemie. ,vol. 449, pp. 102- 108 ,(1926) , 10.1002/JLAC.19264490107
Ronald T. Borchardt, Chao Fu Cheng, Purification and characterization of rat heart and brain catechol methyltransferase Biochimica et Biophysica Acta. ,vol. 522, pp. 49- 62 ,(1978) , 10.1016/0005-2744(78)90321-2
Ronald T. Borchardt, Dhiren R. Thakker, Affinity labeling of catechol O-methyltransferase by N-haloacetyl derivatives of 3,5-dimethoxy-4-hydroxyphenylethylamine and 3,4-dimethoxy-5-hydroxyphenylethylamine. Kinetics of inactivation. Biochemistry. ,vol. 14, pp. 4543- 4551 ,(1975) , 10.1021/BI00691A033
Ronald T. Borchardt, Chao-Fu Cheng, Dhiren R. Thakker, Purification of catechol-O-methyltransferase by affinity chromatography Biochemical and Biophysical Research Communications. ,vol. 63, pp. 69- 77 ,(1975) , 10.1016/S0006-291X(75)80012-X
Richard M. Weinshilboum, Frederick A. Raymond, Calcium inhibition of rat liver catechol-O-methyltransferase☆ Biochemical Pharmacology. ,vol. 25, pp. 573- 579 ,(1976) , 10.1016/0006-2952(76)90390-7
J. W. Daly, C. R. Creveling, B. Nikodejevic, S. Senoh, CATECHOL-O-METHYLTRANSFERASE. II. A NEW CLASS OF INHIBITORS OF CATECHOL-O-METHYLTRANSFERASE; 3,5-DIHYDROXY-4-METHOXYBENZOIC ACID AND RELATED COMPOUNDS Journal of Pharmacology and Experimental Therapeutics. ,vol. 174, pp. 83- 93 ,(1970)