Regulation of mRNA Levels by Decay-Promoting Introns that Recruit the Exosome Specificity Factor Mmi1
作者： Cornelia Kilchert , Sina Wittmann , Monica Passoni , Sneha Shah , Sander Granneman
DOI: 10.1016/J.CELREP.2015.11.026
关键词:
摘要: In eukaryotic cells, inefficient splicing is surprisingly common and leads to the degradation of transcripts with retained introns. How pre-mRNAs are committed nuclear decay unknown. Here, we uncover a mechanism by which specific intron-containing targeted for in fission yeast. Sequence elements within these "decay-promoting" introns co-transcriptionally recruit exosome specificity factor Mmi1, induces unspliced precursor reduction levels spliced mRNA. This negatively regulates RNA helicase DDX5/Dbp2 promote cell survival response stress. contrast, fast removal decay-promoting co-transcriptional precludes Mmi1 recruitment relieves negative expression regulation. We propose that facilitate regulation gene expression. Based on identification multiple additional targets, including mRNAs, long non-coding RNAs, sn/snoRNAs, suggest general role through transcript degradation.
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