New method for culture of zona-included or zona-free embryos: the Well of the Well (WOW) system.
作者： G. Vajta , T.T. Peura , P. Holm , A. P�ldi , T. Greve
DOI: 10.1002/(SICI)1098-2795(200003)55:3<256::AID-MRD3>3.0.CO;2-7
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摘要: Culture of mammalian zygotes individually and in small groups results lower developmental rates than culture large groups. Zona-free also have impaired potential current systems. This paper describes a new approach to resolve the problems, Well (WOW) system. Small wells (WOWs) were formed four-well dishes by melting bottom with heated steel rods. The WOWs then rinsed, filled medium, embryos placed into WOWs. To test value WOW system 3 × factorial experiment was performed. Bovine presumptive cultured from day 1 7 (day 0: insemination) using three modules (single embryos, embryo five, or single zona-digested embryos) different systems (400 μl 200 drops, WOWs). An additional control group consisted 40 50 400 medium. resulted higher blastocyst/oocyte for all (single: 59%; five: 61%; zona-digested: 53%) drops (P < 0.05 all). rate independent number per well. cell blastocysts did not differ that controls. Apart its theoretical revealing role factors influencing development vitro, may immediate practical consequences certain areas production. Mol Reprod Dev 55:256–264, 2000. © 2000 Wiley-Liss, Inc.
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