Gestational Form of Supplemental Selenium (Se) Affects Steroidogenic Gene Expression in the Newborn Calf Testis

摘要: OF THE THESIS GESTATIONAL FORM SUPPLEMENTAL SELENIUM (SE) AFFECTS STEROIDOGENIC GENE EXPRESSION IN NEWBORN CALF TESTIS Selenium (Se) is an important trace mineral in the diet of cattle. Our objective was to determine whether form supplemental Se fed dam would affect expression genes regulating steroidogenesis newborn testis. Twenty-four Anguscross cows were assigned randomly (n=8) individual ad libitum access a mix containing 35 ppm supplied as sodium selenite (inorganic, ISe; Prince Se), SelPlex (organic, OSe; Sel-Plex, Alltech) or 50/50 ISe/OSe (MIX) 4 months prior breeding and throughout gestation. All male calves castrated within 2 days birth total testis RNA subjected microarray analysis using Affymetrix Bovine 1.0 ST array. Ingenuity Pathway Analysis differentially expressed genes, separated by one-way ANOVA (p<0.05) post-hoc LSD test, identified eight mRNAs associated with steroidogenesis. Specifically, involved conversion testosterone estradiol increased OSe-supplemented when compared ISe-supplemented animals, encoding androstenedione decreased MIX-supplemented cows. Expression neonate may equate differences fertility adult animal.