Microsatellite-based genetic relationships in the genus Camellia: potential for improving cultivars.
作者： Matteo Caser , Daniela Torello Marinoni , Valentina Scariot
DOI: 10.1139/G10-012
关键词:
摘要: There is a lack of published microsatellite data that characterizes Camellia spp. To address this, an initial study sequence tagged site (STMS) variation was undertaken with 132 accessions spp., which included 24 representing 22 different species or varieties as well 63 cultivars C. japonica, 33 C. sasanqua, 7 C. ×vernalis, 3 C. ×hiemalis, and 2 hybrida. The four primer sets used (MSCJAF37, MSCJAH46, MSCJAF25, MSCJAH38) successfully amplified polymorphic alleles in all the analysed, showing cross-transferability. Overall, 96 were scored. MSCJAH38 primers produced highest number bands (30), while MSCJAH46 yielded lowest (15). genetic distance between pairs estimated on basis Nei coefficient principal coordinate analysis performed. plot revealed main differentiation japonica winter...
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