The calcium silicate/alginate composite: Preparation and evaluation of its behavior as bioactive injectable hydrogels

摘要: In this study, an injectable calcium silicate (CS)/sodium alginate (SA) hybrid hydrogel was prepared using a novel material composition design. CS incorporated into solution and internal in situ gelling induced by the ions directly released from with addition of d-gluconic acid δ-lactone (GDL). The time could be controlled, about 30s to 10 min, varying amounts GDL added. mechanical properties hydrogels different were systematically analyzed. compressive strength 5% CS/SA higher than that 10% for same amount GDL. swelling behaviors contents therefore investigated. ratios decreased increasing GDL, 1% swelled only less 5%. Scanning electron microscopy (SEM) observation scaffolds showed optimal interconnected porous structure, pore size ranging between 50 200 μm. Fourier transform infrared spectroscopy SEM composite formation hydroxyapatite on surface materials simulated body fluid. addition, rat bone mesenchymal stem cells (rtBMSCs) cultured presence their ionic extracts able maintain viability proliferation. Furthermore, its stimulated rtBMSCs produce alkaline phosphatase, also promote angiogenesis human umbilical vein endothelial cells. Overall, all these results indicate efficiently supported adhesion, proliferation differentiation osteogenic angiogenic Together three-dimensional structure properties, possesses great potential regeneration tissue engineering applications.