Zebrafish as a Potential Model Organism for Drug Test Against Hepatitis C Virus

摘要: Screening and evaluating anti- hepatitis C virus (HCV) drugs in vivo is difficult worldwide, mainly because of the lack suitable small animal models. We investigate whether zebrafish could be a model organism for HCV replication. To achieve NS5B-dependent replication an sub-replicon was designed created with two vectors, one ns5b fluorescent rfp genes, other containing HCV's 5′UTR, core, 3′UTR gfp genes. The vectors sub-replicons were co-injected into zygotes. amplified liver showing significant expression core RNA protein. amplification caused no abnormality development growth larvae, but induced gene change similar to that human hepatocytes. As fluorescence live detectable microscopically, it rendered us advantage select those replicating drug experiments. Ribavirin oxymatrine, known anti-HCV drugs, inhibited this reduced levels Technically, method had good reproducibility easy operate. Thus, might host HCV, zebrafish/HCV (sub-replicon) system screening evaluation.