The Animal Model Determines the Results of Aeromonas Virulence Factors.

摘要: The selection of an experimental animal model is great importance in the study bacterial virulence factors. Here, a bath infection zebrafish larvae proposed as alternative to factors A. hydrophila. Intraperitoneal infections mice and trout were compared with using specific mutants. advantage this that immersion mimics natural route infection, injury tail also provides portal entry for bacteria. implication T3SS hydrophila was analysed AH-1::aopB mutant. This mutant less virulent than wild-type strain when inoculated into larvae, described other vertebrates. However, exhibited slight differences mortality kinetics only observed invertebrate models. Infections AH-1∆vapA lacking gene coding surface S-layer suggested protein not totally necessary bacteria once it inside host, but contributed inflammatory response. Only healthy infected did produce wild type. Variations between models evidenced AH-1∆rmlB, which lacks O-antigen lipopolysaccharide (LPS), AH-1∆wahD, LPS part outer-core. Both mutants showed decreased all models, them injured suggesting residues from outer core must be important virulence. greatest AH-1ΔFlaB-J (lacking polar flagella unable swim) AH-1::motX (non-motile producing flagella). They pathogenic injected trout, no mortalities registered larvae. demonstrates can used host assess revealed more pathogenicity vitro enabled detection variations pathogenesis intraperitoneal injections or fish.