Analysis by capillary electrophoresis of the proteolytic activity of a Bacillus subtilis neutral protease on bovine caseins
作者： Silvia M. Albillos , María D. Busto , Manuel Perez-Mateos , Natividad Ortega
DOI: 10.1016/J.IDAIRYJ.2007.02.003
关键词:
摘要: Abstract Capillary electrophoresis using an untreated fused-silica capillary and a low-pH buffer containing urea hydroxypropyl methyl cellulose was used to follow the proteolytic action of neutral protease Bacillus subtilis on isolated casein fractions whole milk (CN) samples. Electrophoretic analysis showed that this caused intense hydrolysis α -CN, β -CN κ -CN. The breakdown products due attack γ 2 A, 1 B, A , 3 -CN-I s s1 -CN-I, s0 f(1–23) were separated.
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