Distinct Na+/K+/2Cl- cotransporter localization in kidneys and gills of two euryhaline species, rainbow trout and killifish
作者： Fumi Katoh , Regina R. F. Cozzi , William S. Marshall , Greg G. Goss
DOI: 10.1007/S00441-008-0679-4
关键词:
摘要: The kidney is an organ playing important role in ion regulation both freshwater (FW) and seawater (SW) fish. mechanisms of the fish are less well studied than that their gills, especially at level transporter proteins. We have found striking differences pattern Na+/K+/2Cl- cotransporter (NKCC) expression between species. In killifish kidney, NKCC apically localized distal collecting tubules basolaterally proximal tubules. However, SW gill, co-localized with Na+/K+-ATPase, whereas FW, immunoreactivity primarily apical, although still colocalized within same mitochondria-rich cell basolateral Na+/K+-ATPase. Rainbow trout has only apical membrane environments, no signal being detected tubule. On other hand, FW environments. An observation that, gills rainbow trout, trailing edge filament possesses mostly Na+/K+-ATPase-positive but NKCC-negative cells, region roots gill lamellae, most cells exhibit Na+/K+-ATPase- NKCC-positive immunoreactivity. These results suggest differential localization transporters two species represents function these euryhaline fishes different life histories strategies.
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