Maximizing gene expression on a plasmid using recombination in vitro
作者： Keith Backman , Mark Ptashne
DOI: 10.1016/0092-8674(78)90138-1
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摘要: Recombination in vitro has been used to place one or more copies of a strong promoter, the lac at varying distances from cl (repressor) gene bacteriophage lambda on E. coli plasmid pMB9. In all constructions, repressor synthesis is driven wholly predominantly by inserted promoter. One our fusions directs very high levels repressor. this case, fused DNA encodes ribosome binding site which "hybrid" and sequences. principle, method construction should elicit expression any gene, whatever its source. We also described strains with different sequence arrangements that, for reasons not completely understood, produce less
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