In vivo imaging method to distinguish acute and chronic inflammation.

摘要: Inflammation is a fundamental aspect of many human diseases. In this video report, we demonstrate non-invasive bioluminescence imaging techniques that distinguish acute and chronic inflammation in mouse models. With tissue damage or pathogen invasion, neutrophils are the first line defense, playing major role mediating inflammatory response. As reaction progresses, circulating monocytes gradually migrate into site injury differentiate mature macrophages, which mediate promote repair by removing debris producing anti-inflammatory cytokines. Intraperitoneal injection luminol (5-amino-2,3-dihydro-1,4-phthalazinedione, sodium salt) enables detection largely mediated tissue-infiltrating neutrophils. Luminol specifically reacts with superoxide generated within phagosomes since results from myeloperoxidase (MPO) reaction. Lucigenin (bis-N-methylacridinium nitrate) also order to generate bioluminescence. However, lucigenin independent MPO it solely relies on phagocyte NADPH oxidase (Phox) macrophages during inflammation. Together, allow visualization longitudinal assessment different populations across both phases. Given important variety diseases, believe method can help investigate differential roles pathological conditions.