Distinct Roles for CBP and p300 on the RA-Mediated Expression of the Meiosis Commitment Gene Stra8 in Mouse Embryonic Stem Cells
作者： Wen Chen , Wenwen Jia , Kai Wang , Xiaoxing Si , Songcheng Zhu
DOI: 10.1371/JOURNAL.PONE.0066076
关键词:
摘要: In mammalian germ cells, meiotic commitment requires the expression of Stimulated by retinoic acid gene 8 (Stra8), which is transcriptionally activated (RA). However, little known about epigenetic mechanism RA induces Stra8 expression. Utilizing a chromatin immunoprecipitation assay (ChIP), we showed that increases histone acetylation at promoter in murine embryonic stem cells (ESCs), model for cell differentiation. Furthermore, explored whether two coregulators with acetyltransferase (HAT) activity, Creb-binding protein (CBP) and p300, are involved activation Stra8. The lentiviral shRNA knockdown endogenous CBP led to repression, while overexpression enhanced both mRNA levels. ChIP analysis confirmed crucial coactivator RA-mediated transcription it enhances level recruits RNA polymerase II establish active chromatin. p300 expression, reduced independently its HAT activity. significantly increased promoter. These findings demonstrate play distinct roles transcription.
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