Early Diagnostic Method of Avian Influenza Virus Subtype Using Ultra Real-Time PCR
作者： Jang Su Kim , Young Kyoon Kim , Sang-Tae Kim
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摘要: This ultra real-time PCR (UPCR) based diagnosis system for avian influenza A virus (AIV) subtype was designed. The target primer in this study derived from H5N1 subtype-specific 133 bp partial gene of hemagglutinin (HA), and synthesized by using PCR-based synthesis on the ground safety. UPCR operated Mini-Opticon Q-PCR Quantitative Thermal Cycler aptamer-based molecular beacon, total 10 μl reaction mixture with extraordinarily short time each steps PCR. detection including analysis melting temperature totally within 15 min. AIV-specific product correctly amplified until 5 molecules HA as minimum templates. kind drafted it could be used to detect not only AIV subtype, but also other pathogens UPCR-based diagnosis.
koreascience.or.kr参考文章(26)
Byoung-Su Yoon, Eul-Hwan Kim, Dong-Woo Lee, Soon-Hwan Kwon, Sang-Hoon Han, Rapid Detection Method of Avian Influenza Subtype H5N1 using Quick Real-Time PCR Korean Journal of Microbiology. ,vol. 43, pp. 23- 30 ,(2007)
A Criado-Fornelio, A review of nucleic-acid-based diagnostic tests for Babesia and Theileria, with emphasis on bovine piroplasms. Parassitologia. ,vol. 49, pp. 39- 44 ,(2007)
M R Castrucci, Y Kawaoka, Biologic importance of neuraminidase stalk length in influenza A virus. Journal of Virology. ,vol. 67, pp. 759- 764 ,(1993) , 10.1128/JVI.67.2.759-764.1993
E. Basuno, Y. Yusdja, N. Ilham, Socio-economic impacts of avian influenza outbreaks on small-scale producers in Indonesia Transboundary and Emerging Diseases. ,vol. 57, pp. 7- 10 ,(2010) , 10.1111/J.1865-1682.2010.01121.X
Toshihiro Ito, Yasuo Suzuki, Lyndon Mitnaul, Angela Vines, Hiroshi Kida, Yoshihiro Kawaoka, Receptor specificity of influenza A viruses correlates with the agglutination of erythrocytes from different animal species Virology. ,vol. 227, pp. 493- 499 ,(1997) , 10.1006/VIRO.1996.8323
Anthony S. Fauci, Emerging and re-emerging infectious diseases: influenza as a prototype of the host-pathogen balancing act. Cell. ,vol. 124, pp. 665- 670 ,(2006) , 10.1016/J.CELL.2006.02.010
M. Ben Shabat, R. Meir, R. Haddas, E. Lapin, I. Shkoda, I. Raibstein, S. Perk, I. Davidson, Development of a real-time TaqMan RT-PCR assay for the detection of H9N2 avian influenza viruses Journal of Virological Methods. ,vol. 168, pp. 72- 77 ,(2010) , 10.1016/J.JVIROMET.2010.04.019
Zheng Li, Chi Ma, Zhonghua Liu, Wei He, Serologic cross-reactivity among humans and birds infected with highly pathogenic avian influenza A subtype H5N1 viruses in China. Immunology Letters. ,vol. 135, pp. 59- 63 ,(2011) , 10.1016/J.IMLET.2010.09.012
Sung Ho Jeon, Basak Kayhan, Tamar Ben-Yedidia, Ruth Arnon, A DNA aptamer prevents influenza infection by blocking the receptor binding region of the viral hemagglutinin. Journal of Biological Chemistry. ,vol. 279, pp. 48410- 48419 ,(2004) , 10.1074/JBC.M409059200
F.X. Bosch, M. Orlich, H.-D. Klenk, R. Rott, The structure of the hemagglutinin, a determinant for the pathogenicity of influenza viruses Virology. ,vol. 95, pp. 197- 207 ,(1979) , 10.1016/0042-6822(79)90414-8