Temperature-sensitive mutants of p53 homologs.
作者： Radhika Pochampally , Changgong Li , Wenge Lu , Lihong Chen , Ronald Luftig
关键词:
摘要: Two homologs of the p53 tumor suppressor, p63 and p73 have recently been discovered. These proteins activities similar to in cell culture but distinct developmental functions vivo. We found that temperature-sensitive mutants certain isoforms can be created by single amino acid substitutions an alanine residue corresponding 135 murine p53. The (p63γ-Pro167, p73α-Leu156 p73β-Ile156) controlled temperature shift between 32°C 39°C. They stably expressed p53-null H1299 cells at 39°C, become transcriptionally activated 32°C, induce expression p53-responsive genes MDM2 p21WAF1. Activation p73β-Ile156 inhibits division induces significant increase size (hypertrophy), whereas activation p63γ-Pro167 apoptosis. may useful tools for gaining further insight homologs.
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