Integrated ethanol biosensors based on carbon paste electrodes modified with [Re(phen-dione)(CO)3Cl] and [Fe(phen-dione)3](PF6)2

摘要: Abstract The preparation and electrochemical characterization of carbon paste electrodes modified with 1,10-phenantroline-5,6-dione (phen-dione) complexes Fe ([Fe(phen-dione)3]2+) Re ([Re(phen-dione)(CO)3Cl]) as well their behavior electrocatalysts towards the oxidation NADH are described. These exhibit redox responses very similar to those in aqueous solution, including pH-dependent response ascribed pendant o-quinone groups, observed for films electrodeposited onto glassy electrodes. Carbon either complexes, exhibited potent persistent electrocatalytic activity at potentials around 0.0 V versus SSCE, which represents a dramatic diminution overpotential. Using complex, alcohol dehydrogenase NAD+ we have developed an integrated ethanol biosensor. It detection limit 45 μM (S/N = 3), linearity up 4.0 mM, sensitivity 174 nA mM−1 stability least 8 days when stored 4°C.