Bacillus subtilis generates a major specific deletion in pAM beta 1.

摘要: Abstract pAM beta 1, a 26.5-kilobase plasmid originally isolated from Streptococcus faecalis, was conjugally transferred lactis to Bacillus subtilis. No conjugal transfer of pAM 1 B. subtilis S. observed. In addition, which had been reintroduced in after cycling through lost its transferability cremoris, although under the same conditions noncycled at high efficiency. Restriction and Southern blot analyses showed that suffered one major, specific 10.6-kilobase deletion several minor but also deletions Comparing major derivative, delta with strains have reported contain these contained 1. Hybridization experiments deleted fragment not transposed chromosome. Based on size derivatives it is suggested use different origin replication