Curcumin inhibits cell growth and induces cell apoptosis through upregulation of miR-33b in gastric cancer.

摘要: In this work, the in vitro experiments about biological mechanisms of curcumin were conducted using gastric cancer cell lines SGC-7901 and BGC-823. After 24-h exposure to at concentrations 5, 10, 15, 20, 40 μmol/L, two cells showed decreased proliferation increased apoptosis abilities. Real-time PCR, Cell Counting Kit-8 (CCK-8) assay, western blotting, assay used further study underlying curcumin. The first stage our studies that affected expression miR-33b, which, turn, X-linked inhibitor protein (XIAP) messenger RNA (mRNA). Next, was also identified regulate BGC-823 cells. Further bioinformatics analysis luciferase reporter assays proved XIAP one target genes miR-33b. next stage, treated with 20 μL curcumin, miR-33b mimics, small interfering (siRNA) XIAP, respectively. results had similar effects on growth as upregulation siRNA XIAP. followed from restore presumably by upregulating targeting cancer. Collectively, indicate curcumin-miR-33b-XIAP coupling might be an important mechanism which induces