Speciation of inorganic selenium and selenoaminoacids by on-line reversed-phase high-performance liquid chromatography–focused microwave digestion–hydride generation-atomic detection

摘要: A high-performance liquid chromatographic–microwave digestion–hydride generation system coupled on-line with three atomic detectors (atomic absorption, inductively plasma emission and mass spectrometry) has been developed investigated for selenium species separation determination. Total inorganic selenium, selenomethionine selenoethionine are separated by reversed-phase chromatography prior to microwave digestion of selenocompounds a KBrO3–HBr mixture form continuously SeIV, which is finally transformed into H2Se, also in continuous manner, merging flow sodium borohydride. Detection limits obtained each Se water urine using the have worked out compared (i.e., SeIV DLs were 6.8 µg l–1 AAS, 30 ICP-AES 0.16 ICP-MS).The integrated system, HPLC–MW digestion–HG-atomic detection, proposed here allows, single injection, reliable speciation selenoaminoacids tested versus total selenium. Further overlapped SeVI peaks accomplished second injection sample determine only avoiding heating. Results on human shown that more information actual species, perhaps unknown, present real samples could be gathered resorting use two, or more, same scheme.