A stereological analysis of the effect of buffer osmolarity on a salt secreting epithelium, under variable physiological conditions

摘要: SUMMARY A stereological analysis of salt gland epithelium showed differences in the size intracellular space and volume fraction some cellular organelles, according to salinity which animals were kept. These changes similar earlier non-quantitative work on several secreting epithelia various states activity. However, alteration one variable processing technique, osmolarity fixative buffer, was shown produce same result glands held at constant activity. Most experimental manipulations used previous experiments alter glandular activity, e.g. loading, altered perfusate, or inclusion pump inhibitors, probably also ion contents. It is therefore difficult decide whether lateral intercellular geometry represent physiological mechanisms vivo osmotic behaviour during for electron microscopy.