Characterization of nuclear RNA synthesis in Amoeba proteus.

摘要: Abstract The synthesis of RNA in the ameba nucleus has been studied by labeling with 3H-uridine and fractionation sucrose density gradients. After a relatively short period radioactivity is primarily restricted to 30S, 19S, (both ribosomal) 4S regions, no significant amount detectable regions above Various tests appear exclude possibility differential breakdown or loss larger than 30S during preparative procedures. We conclude that does not synthesize high molecular weight, rapid turnover, intranuclear (HnRNA) found variety metazoans possibly pre-ribosomal RNA. In search literature we could find convincing evidence for HnRNA unicellular eukaryotes. It appears line separation occurrence may be between prokaryotes eukaryotes but rather multicellular Experiments employing long term labeling, followed “chase”, starvation labeled cells demonstrate long-lived nucleus. This gradient, although amounts are present region these size classes. studies prominent 19S peak nuclear observed virtually absent, indicating as other much more exit from comparison region.