Cloning of a gene (SR-A1), encoding for a new member of the human Ser/Arg-rich family of pre-mRNA splicing factors: overexpression in aggressive ovarian cancer.
作者： A Scorilas , L Kyriakopoulou , D Katsaros , E P Diamandis
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摘要: By using the positional cloning gene approach, we were able to identify a novel encoding for serine/arginine-rich protein, which appears be human homologue of rat A1 gene. We named this new SR-A1. Members SR family proteins have been shown interact with C-terminal domain (CTD) large subunit RNA polymerase II and participate in pre-mRNA splicing. localized SR-A1 between known genes IRF3 RRAS on chromosome 19q13.3. The spans 16.7 kb genomic sequence it is formed 11 exons 10 intervening introns. protein composed 1312 amino acids, molecular mass 139.3 kDa theoretical isoelectric point 9.31. contains an SR-rich as well CTD-binding present only subset SR-proteins. Through interactions CTD Polymerase II, regulate alternative expressed all tissues tested, highest levels found fetal brain liver. Our data suggest that overexpressed ovarian cancers are clinically more aggressive. Studies steroid hormone receptor-positive breast prostate carcinoma cell lines ZR-75-1, BT-474 LNCaP, respectively, constitutively expressed. Furthermore, mRNA these increase by estrogens, androgens glucocorticoids, lesser extend progestins.
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