The unravelling of the complex pattern of tyrosinase inhibition.

摘要: Tyrosinases are responsible for melanin formation in all life domains. Tyrosinase inhibitors used the prevention of severe skin diseases, skin-whitening creams and to avoid fruit browning, however continued use many such is considered unsafe. In this study we provide conclusive evidence inhibition mechanism two well studied tyrosinase inhibitors, KA (kojic acid) HQ (hydroquinone), which extensively hyperpigmentation treatment. reported literature with contradicting mechanisms, while described as both a inhibitor substrate. By visualization active site TyrBm crystals, together molecular modeling, binding constant analysis kinetic experiments, have elucidated their mechanisms inhibition, was ambiguous inhibitors. We confirm that acts mixed inhibitor, can act substrate an inhibitor.