Detection Of Hepatitis C Virus (Hcv) Infection And Its Genotype In Patients At Hepatology Outpatient Clinic, Dr. Soetomo General Hospital, Surabaya

摘要: Hepatitis C virus (HCV) is an RNA that can cause liver inflammation (hepatitis) and has the potential to become chronic progress cirrhosis hepatocellular carcinoma. Detection of HCV infection, genotype/subtype was performed on 70 blood sera patients at Hepatology Outpatient Clinic Dr Soetomo General Hospital, Surabaya, Indonesia. infection carried out by Anti-HCV determination using enzyme immunoassay (EIA) technique, detection Reverse Transcription Polymerase Chain Reaction (RT-PCR) technique based genome regions NS5b 5'UTR, followed electrophoresis with agarose gel. In positive PCR results, determined direct sequencing method ABI 310 sequencer results were analyzed comparing products previously published nucleotides. Sera obtained from 41 (58.6%) male 29 (41.4%) female patients. found in 17/70 (24.29%) 16/17 (94.1%) proved contain when RT-PCR technique. Patients have transmit infection. From analysis we 2/16 (12,5%), 3/16 (18,75%) 6/16 (37,5%), 1/16 (6,25%), (6, 25%), (6,25%) genotypes 1, 2, subtypes 1b, 1c, 2a, 3a, 3k respectively.Conclusion: who went Clinic, Dr. Hospital 24,29%. 94,1% Anti-HCV, still detected genotype 1 subtype 1b dominant subtypes.