Specificity of Mimotope-Induced Anti-High Molecular Weight-Melanoma Associated Antigen (HMW-MAA) Antibodies Does Not Ensure Biological Activity

摘要: Vaccines based on peptide mimics (mimotopes) of conformational tumor antigen epitopes have been investigated for a variety human tumors including breast cancer, expressing the carcinoembryonic antigen, B cell lymphoma, neuroblastoma, and melanoma. In our previous work, we designed vaccine mimotope high molecular weight-melanoma associated (HMW-MAA) that elicited HMW-MAA-specific antibodies (Abs) with anti-tumor activity in vitro vivo. this study, aimed to identify mimotopes additional distinct HMW-MAA epitopes, since they could be used construct polymimotope melanoma vaccine. For purpose, random phage libraries were screened anti-HMW-MAA monoclonal (mAbs) VT80.12 VF1-TP43 yielding one ligand each mAb. Both peptides inhibited binding corresponding mAb HMW-MAA. Furthermore, when coupled carrier protein keyhole limpet hemocyanin (KLH), both peptide-specific Abs rabbits or BALB/c mice, but only isolated mice. However, latter had no detectable effect cells vitro. These results describe limitations related display technique emphasize need characterize functional properties utilized isolate epitopes.