Cancer Stem Cell-Targeted Gene Delivery Mediated by Aptamer-Decorated pH-Sensitive Nanoliposomes.
作者： Parikshit Moitra , Parikshit Moitra , Phuong Tran , Wei Duan , Paturu Kondaiah
DOI: 10.1021/ACSBIOMATERIALS.1C00110
关键词:
摘要: A new pH-responsive cationic co-liposomal formulation was prepared in this study using the twin version of amphiphile palmitoyl homocysteine, TPHC; natural zwitterionic lipid, DOPE; and cholesterol-based C5C, at specified molar ratios. This co-liposome further decorated with a newly designed fluorescently tagged, cholesterol-tethered EpCAM-targeting RNA aptamer for targeted gene delivery. aptamer-guided nanoliposomal formulation, C5C/DOPE/TPHC 8:24:1 ratio, could efficiently transport genes response to low pH cellular endosomes selectively EpCAM overexpressing cancer stem cells. particular observation extended siRNA against GFP validate their transfection capabilities expression. Overall, found be an excellent transfectant vitro
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