Hydrothermal carbonization of livestock mortality for the reduction of pathogens and microbially-derived DNA

摘要: Hydrothermal carbonization (HTC), utilizing high temperature and pressure, has the potential to treat agricultural waste via inactivating pathogens, antibiotic resistance genes (ARG), contaminants of emerging concern (CEC) in a environmental economical manner. Livestock mortality is one facet that can pose threat surrounding environment. While several methods are utilized livestock mortality, there remains paucity data on elimination microbially-derived DNA these treatment practices. This DNA, most notably ARGs, if it survives be reintroduced environments where could potentially passed posing risk animal human populations. HTC treatments have been successfully for CECs, however very little understood how ARGs survive treatment. study aims fill this knowledge gap by examining survivability mortality. We examined three temperatures (100°C, 150°C, 200° C) at autogenic pressures times (30, 60, 240 min). amplification plasmid-borne reporter gene carried Escherichia coli DH10B introduced both beef bone tissue. Results indicate while all temperatures, times, were suitable complete pathogen kill, only 150°C 200°C sufficient eliminating microbial DNA. These results serve as basis future recommendations when considering pathogens ARGs. Open image new window