Neuroprotective effects of Tacrolimus (FK-506) and Cyclosporin (CsA) in oxidative injury.
作者： Seema Yousuf , Fahim Atif , Varun Kesherwani , Sandeep Kumar Agrawal
DOI: 10.1002/BRB3.16
关键词:
摘要: The detrimental effects of hypoxic damage to central nervous system lead energy depletion, free radical formation, lipid peroxidation (LPO), and increased calcium. We hypothesized that in vitro tacrolimus (FK-506) cyclosporine A (CsA) could be protective against spinal cord. Dorsal columns were isolated from the cord adult rats injured by exposure condition for 1 h, treated with FK-506 (0.1 μM) CsA μM). After injury, reperfusion was carried out 2 h. Tissues collected, processed biochemical assays, 2,3,5-triphenyltetrazolium chloride (TTC) staining. Spinal hypoxia caused a significant decrease (P < 0.001) mitochondrial ATP (30.64%) tissue reduced glutathione (GSH) (60.14%) content. Conversely, increase LPO level (57.77%) myeloperoxidase (MPO) activity (461.24%) observed group. Mitochondrial swelling also significantly group (90.0%). Treatment either or showed neuroprotective 0.05–0.01) measured various parameters groups. treatment 11.19% 16.14% while GSH content 66.46% 77.32%, respectively. decreased 18.97% 24.06% MPO 42.86% 18.66% after treatment. Calcium uptake mitochondria as exhibited absorbance TTC staining viability In conclusion, present study demonstrates effect induced is mediated via their antioxidant actions.
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sci-hub.se 参考文章(39)
Marina Aksenova, D. Allan Butterfield, Shu-Xin Zhang, Mark Underwood, James W. Geddes, Increased protein oxidation and decreased creatine kinase BB expression and activity after spinal cord contusion injury. Journal of Neurotrauma. ,vol. 19, pp. 491- 502 ,(2002) , 10.1089/08977150252932433
PP Bradley, RD Christensen, G Rothstein, Cellular and Extracellular Myeloperoxidase in Pyogenic Inflammation Blood. ,vol. 60, pp. 618- 622 ,(1982) , 10.1182/BLOOD.V60.3.618.618
A P Halestrap, A M Davidson, Inhibition of Ca2+-induced large-amplitude swelling of liver and heart mitochondria by cyclosporin is probably caused by the inhibitor binding to mitochondrial-matrix peptidyl-prolyl cis-trans isomerase and preventing it interacting with the adenine nucleotide translocase Biochemical Journal. ,vol. 268, pp. 153- 160 ,(1990) , 10.1042/BJ2680153
Andrew Halestrap, A pore way to die Nature. ,vol. 434, pp. 578- 579 ,(2005) , 10.1038/434578A
Marcel Leist, Pierluigi Nicotera, None, Apoptosis, Excitotoxicity, and Neuropathology Experimental Cell Research. ,vol. 239, pp. 183- 201 ,(1998) , 10.1006/EXCR.1997.4026
Jason Sheehan, Anne Eischeid, Randi Saunders, Nader Pouratian, Potentiation of neurite outgrowth and reduction of apoptosis by immunosuppressive agents: implications for neuronal injury and transplantation. Neurosurgical Focus. ,vol. 20, pp. 1- 7 ,(2006) , 10.3171/FOC.2006.20.5.10
Hong-Gang Wang, Nuzhat Pathan, Iryna M Ethell, Stanislaw Krajewski, Yu Yamaguchi, Futoshi Shibasaki, Frank McKeon, Tanya Bobo, Thomas F Franke, John C Reed, Ca2+-induced apoptosis through calcineurin dephosphorylation of BAD. Science. ,vol. 284, pp. 339- 343 ,(1999) , 10.1126/SCIENCE.284.5412.339
E HALL, J BRAUGHLER, Central nervous system trauma and stroke. II. Physiological and pharmacological evidence for involvement of oxygen radicals and lipid peroxidation Free Radical Biology and Medicine. ,vol. 6, pp. 303- 313 ,(1989) , 10.1016/0891-5849(89)90057-9
Dennis W. Choi, Calcium: still center-stage in hypoxic-ischemic neuronal death Trends in Neurosciences. ,vol. 18, pp. 58- 60 ,(1995) , 10.1016/0166-2236(95)80018-W
D. A. Ramsay, G. A. Dekaban, A. E. Marcillo, A. D. Saenz, M. Pasquale-Styles, W. D. Dietrich, L. C. Weaver, J. C. Fleming, M. D. Norenberg, The cellular inflammatory response in human spinal cords after injury. Brain. ,vol. 129, pp. 3249- 3269 ,(2006) , 10.1093/BRAIN/AWL296