Role of petasin in the potential anti-inflammatory activity of a plant extract of petasites hybridus11Abbreviations: AA, arachidonic acid; [Ca2+]i, cytosolic free calcium concentration; cPLA2, cytosolic phospholipase A2; C5a, complement factor C5a; FLAP, 5-LO-activating protein; GM-CSF, granulocyte-macrophage colony-stimulating factor; IL, interleukin; IP3, inositol trisphosphate; 5-LO, 5-lipoxygenase; LT, leukotriene; MAPK, mitogen-activated protein kinase; mAb, monoclonal antibody; PAF, platelet-activating factor; and Ze339, CO2 estract of petasites hybridus.

摘要: Abstract A large production of leukotrienes (LTs) can be induced in human eosinophils or neutrophils by priming with granulocyte-macrophage colony-stimulating factor and subsequent stimulation platelet-activating (PAF) the anaphylatoxin C5a. Here, we investigated effects a plant extract petasites hybridus (Ze339) its isolated active sesquiterpene ester petasin these two vitro cell models. Zileuton, 5-lipoxygenase inhibitor, was used as positive control. All compounds inhibited both cysteinyl-LT synthesis LTB 4 neutrophils. In contrast, only Ze339 petasin, but not zileuton, abrogated PAF- C5a-induced increases intracellular calcium concentrations. These data suggest that may block, compared to earlier signalling events initiated G protein-coupled receptors granulocytes, perhaps at level proximal phospholipase C β . Taken together, appears one major compound extract, since it demonstrates same inhibitory activities on fluxes LT generation does.