FLU: a negative regulator of chlorophyll biosynthesis in Arabidopsis thaliana.

摘要: Tetrapyrroles such as chlorophylls and bacteriochlorophylls play a fundamental role in the energy absorption transduction activities of photosynthetic organisms. Because these molecules, however, organisms are also prone to photooxidative damage. They had evolve highly efficient strategies control tetrapyrrole biosynthesis prevent accumulation free intermediates that potentially extremely destructive when illuminated. In higher plants, metabolic flow is regulated at step δ-aminolevulinic acid synthesis. This regulation previously has been attributed feedback Glu tRNA reductase, first enzyme committed biosynthesis, by heme. With recent discovery chlorophyll acting signals both nuclear gene it seems likely heme not only regulator this pathway. A genetic approach was used identify additional factors involved biosynthesis. Arabidopsis thaliana, we have found negative FLU, which operates independently selectively affect Mg2+ branch The identity protein established map-based cloning sequencing FLU gene. nuclear-encoded plastid that, after import processing, becomes tightly associated with membranes. It unrelated any enzymes known be Its predicted features suggest mediates its regulatory effect through interaction