Modified polyethyleneimine with histidine-lysine short peptides as gene carrier.
作者： M Hashemi , B H Parhiz , A Hatefi , M Ramezani
DOI: 10.1038/CGT.2010.57
关键词:
摘要: There are several strategies that can be utilized to improve transfection efficiency while reducing the cytotoxicity of polyethyleneimine (PEI) as a promising non-viral gene delivery vector. In this study, we evaluated potential use lysine–histidine (KH) peptides in modifying PEI 10 kDa structure and enhancing its maintaining low toxicity PEI. was modified with 6-bromohexanoic acid (alkyl) increase lipophilicity. Then, ethylenediamine (EDA) attached carboxylic groups PEI-hexanoate restore primary amines Subsequently, six different KH short were conjugated PEIs for effect sequence on vector cytotoxicity. The PEI-peptides complexed luciferase reporter (pRLCMV) Neuro-2A murine neuroblastoma cells showed KHHHKKHHHK peptide had significantly higher rate comparison other peptides. This PEI-alkyl PEI-alkyl-EDA significant improvement minimal observed. results obtained suggest content will have impact 10 kDa.
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