Sensitive electrochemical aptasensor by coupling "signal-on'' and "signal-off'' strategies.
作者： Liang Wu , Xiaohua Zhang , Wei Liu , Erhu Xiong , Jinhua Chen
DOI: 10.1021/AC401810T
关键词:
摘要: A simple electrochemical aptasensor for sensitive and selective determination of adenosine triphosphate (ATP) has been developed on the basis of a new dual-signaling amplification strategy. This aptasensor features both ''signal-on''and ''signal-off''elements. The ferrocene (Fc)-labeled aptamer probe (Fc-P) is designed to hybridize with the thiolated methylene blue (MB)-modified DNA probe (MB-P) on gold electrode to form rigid duplex DNA. In the presence of ATP, the interaction between ATP and the aptamer leads to the dissociation of …
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