Efficient large-scale development of microsatellites for marker and mapping applications in Brassica crop species.

摘要: A set of 398 simple sequence repeat markers (SSRs) have been developed and characterised for use with genetic studies Brassica species. Small-insert (250-900 bp) genomic libraries from rapa, B. nigra, oleracea napus, highly enriched dinucleotide trinucleotide SSR motifs, were constructed. Screening the clones a mixture oligonucleotide probes revealed positive hybridisation to between 75% 90% clones. Of these, 1,230 sequenced. Primer pairs designed clones, 270 (67.8%) amplified PCR product expected size in their focal and/or closely related further screen 138 primers that produced napus germplasm found 86 (62.3%) length polymorphisms within at least one line test array representing four The results this used identify 56 SSRs combined 41 had previously shown polymorphism parents mapping population. These 97 mapped relative framework RFLP detected 136 loci over all 19 linkage groups oilseed rape genome.