Determination of variants produced upon replication or transcription of nucleic acid sequences

摘要: A method of determining whether or not a nucleic acid having an expected sequence one more variants the are present in sample containing acids after replication, transcription editing (or other transformation) substrate acid. The involves deciding likely to be formed upon acid, and possible sequence, providing primer pairs for polymerase chain reaction, reverse transcriptase reaction ligase carrying out steps form amplicons, analyzing amplicons and/or sample. primers designed anneal regions variants, being selected reveal unambiguously presence absence thereof according specific amplified by primers.