Evaluation of the expression and function of the P2X7 receptor and ART1 in human regulatory T-cell subsets.

摘要: Abstract Regulatory T cells that express CD39 (CD39+ Treg) exhibit specific immunomodulatory properties. Ectonucleotidase hydrolyses ATP and ADP. is a ligand of the P2X7 receptor induces shedding CD62L apoptosis. However, role in CD39+ Treg has not been defined. Furthermore, NAD can activate via ADP-ribosyltransferase (ART) enzymes cause cell depletion murine models. We evaluated expression function ART1 CD39- presence or absence NAD. isolated peripheral blood mononuclear from healthy subjects purified CD4+ cells, CD25+ cells. was assessed by flow cytometry real-time PCR. Our results showed low on higher levels than other subtypes studied. Neither nor death observed 1mM 60 μM In contrast, P2Xs receptor-dependent proliferation with 300 μM ATP, inhibited different types analysed. The proliferation-inhibition increased A2a agonist reversed antagonist, therefore inhibits P2Xs-dependent activation. conclusion, our suggest altered human CD39− could participate resistance against induced