Method and apparatus for automated electrophoresis using light polarization detector

作者: John K Stevens , Alexandre M Izmailov

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摘要: Changes in polarized light incident on a detection zone with separation matrix are used to detect optically active molecules within the matrix. The and of presence involves: a) loading sample containing onto matrix; b) applying motive force cause migrate through separate into plurality subgroups molecules; c) directing an beam radiation d) processing collected exiting optical component which discriminates between having same polarization as different from beam; e) measuring intensity processed beam, wherein difference standard obtained for absence is indicative molecule zone. Alternatively, alternately left right circularly type generated using Pockels cell Ker can be used. When such encounters substance absorbs wavelength, one preferentially absorbed. Differences pulses that has passed indicate species

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