Expression partitioning of duplicate genes at single cell resolution in Arabidopsis roots

摘要: ABSTRACT Gene duplication is a key evolutionary phenomenon, prevalent in all organisms but particularly so plants, where whole genome (WGD; polyploidy) major force evolution. Much effort has been expended attempting to understand the evolution of duplicate genes, addressing such questions as why some paralogue pairs rapidly return single copy status whereas, other pairs, paralogues are retained and may (or not) diverge expression pattern or function. The effect gene—its site thus initial locus its function—occurs at level cell comprising type given state cell’s development. Thus, it critical duplicated gene cellular resolution. Using Arabidopsis thaliana root transcriptomic data we identify 36 clusters, each representing particular developmental state, analyze patterns over 11,000 produced by three cycles polyploidy well various types mechanisms. We categorize their expression, identifying showing strongly biased paralogue/homoeologue different clusters. Notably, precision cell-level permits identification alternate bias, with 90% greater pair’s consistent subfunctionalization level, and, cases, individual cells. set 7,000 genes whose clusters suggests that ancestor was also expressed With this information hypothesize there have shifts for majority degrees depending, expected, on function type, state.