Isolation and functional characterization of CsLsi1, a silicon transporter gene in Cucumis sativus

作者: Hao Sun , Jia Guo , Yaoke Duan , Tiantian Zhang , Heqiang Huo

DOI: 10.1111/PPL.12515

关键词:

摘要: Cucumber (Cucumis sativus) is a widely grown cucurbitaceous vegetable that exhibits relatively high capacity for silicon (Si) accumulation, but the molecular mechanism uptake remains to be clarified. Here we isolated and characterized CsLsi1, gene encoding transporter in cucumber (cv. Mch-4). CsLsi1 shares 55.70 90.63% homology with Lsi1s of monocot dicot, rice (Oryza sativa) pumpkin (Cucurbita moschata), respectively. was predominantly expressed roots, application exogenous suppressed its expression. Transient expression protoplasts showed localized plasma membrane. Heterologous Xenopus laevis oocytes evidenced influx transport activity not urea or glycerol. Expression CsLsi1-mGFP under own promoter at distal side endodermis cortical cells root tips as well hairs near tips. mutant defective over-expression this further confirmed role uptake. Our results suggest cucumber. The cellular localization roots different from other plants, implying possible effect on capability.

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