Analytical and clinical performance of Abbott RealTime MTB, an assay for detection of Mycobacterium tuberculosis in pulmonary specimens.
作者： Ning Tang , Andrea Frank , Vihanga Pahalawatta , John Lampinen , Anke Coblenz-Korte
DOI: 10.1016/J.TUBE.2015.05.010
关键词:
摘要: Nucleic acid amplification test (NAAT)-based assays provide fast and sensitive results compared to conventional TB tests. The performance of the new Abbott Molecular MTB assay for qualitative detection complex using automated m2000™ system or manual sample preparation is summarized in this paper. detects eight subspecies. observed limit (LOD) when used an H37Rv panel was 2.45 colony forming units (cfu)/mL, while claimed LOD with strain 17 cfu/mL. No cross reactivity, carryover were study. clinical sensitivity 93% overall; 99% smear positive, culture positive specimens, 81% negative, samples. specificity 97%. inhibition rate study 0.34%. data suggest that RealTime a reliable, robust molecular MTB.
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tuberculosisjournal.com 参考文章(11)
Robert W. McKinney, Jonathan Y. Richmond, Biosafety in microbiological and biomedical laboratories U.S. G.P.O. : For sale by the Supt. of Docs., U.S. G.P.O.. ,(1999)
Chao Qi, Carole Wallis, Vihanga Pahalawatta, Andrea Frank, Neeshan Ramdin, Raquel Viana, Klara Abravaya, Gregor Leckie, Ning Tang, Evaluation of the efficiency of the sample inactivation reagent in the Abbott RealTime MTB assay for inactivation of Mycobacterium tuberculosis Journal of Clinical Microbiology. ,vol. 53, pp. 3001- 3002 ,(2015) , 10.1128/JCM.00598-15
A B Andersen, E B Hansen, Structure and mapping of antigenic domains of protein antigen b, a 38,000-molecular-weight protein of Mycobacterium tuberculosis Infection and Immunity. ,vol. 57, pp. 2481- 2488 ,(1989) , 10.1128/IAI.57.8.2481-2488.1989
D. Thierry, M.D. Cave, K.D. Eisenach, J.T. Crawford, J.H. Bates, B. Gicquel, J.L. Guesdon, IS6110, an IS-like element of Mycobacterium tuberculosis complex. Nucleic Acids Research. ,vol. 18, pp. 188- 188 ,(1990) , 10.1093/NAR/18.1.188
Haileyesus Getahun, Christian Gunneberg, Reuben Granich, Paul Nunn, HIV Infection—Associated Tuberculosis: The Epidemiology and the Response Clinical Infectious Diseases. ,vol. 50, ,(2010) , 10.1086/651492
Ning Tang, Shihai Huang, John Salituro, Wai-Bing Mak, Gavin Cloherty, Julie Johanson, Yu Hong Li, George Schneider, John Robinson, John Hackett, Priscilla Swanson, Klara Abravaya, A RealTime HIV-1 viral load assay for automated quantitation of HIV-1 RNA in genetically diverse group M subtypes A–H, group O and group N samples Journal of Virological Methods. ,vol. 146, pp. 236- 245 ,(2007) , 10.1016/J.JVIROMET.2007.07.003
A Hesseling, P D van Helden, G K Chege, E Engelke, R M Warren, N C Gey van Pittius, T C Victor, E G Hoal, M C Gutierrez, M de Kock, M Barnard, Differentiation of Mycobacterium tuberculosis complex by PCR amplification of genomic regions of difference. International Journal of Tuberculosis and Lung Disease. ,vol. 10, pp. 818- 822 ,(2006)
Barun Mathema, Natalia E. Kurepina, Pablo J. Bifani, Barry N. Kreiswirth, Molecular Epidemiology of Tuberculosis: Current Insights Clinical Microbiology Reviews. ,vol. 19, pp. 658- 685 ,(2006) , 10.1128/CMR.00061-05
Kathleen A. Alexander, Pete N. Laver, Anita L. Michel, Mark Williams, Paul D. van Helden, Robin M. Warren, Nicolaas C. Gey van Pittius, Novel Mycobacterium tuberculosis complex pathogen, M. mungi Emerging Infectious Diseases. ,vol. 16, pp. 1296- 1299 ,(2010) , 10.3201/EID1608.100314
C. M. Gilpin, D. J. Dawson, G. O'Kane, J. G. Armstrong, C. Coulter, Failure of Commercial Ligase Chain Reaction To Detect Mycobacterium tuberculosis DNA in Sputum Samples from a Patient with Smear-Positive Pulmonary Tuberculosis Due to a Deletion of the Target Region Journal of Clinical Microbiology. ,vol. 40, pp. 2305- 2307 ,(2002) , 10.1128/JCM.40.6.2305-2307.2002